Peptide Storage
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Best Practices For Storing Peptides
To preserve the integrity of laboratory results, proper storage of peptides is essential. Correct storage practices can maintain peptides for years and guard against contamination, oxidation, and degradation that may render your peptides, and therefore experiments, useless. While some peptides are more susceptible to degradation than others, knowing and implementing the best practices for peptide storage can greatly lengthen their stability and integrity regardless of composition.
Short-Term Storage
If the peptides will be used immediately, or in the next several days, weeks or months, short-term refrigeration under 4C (39F) is generally acceptable. Lyophilized peptides are usually stable at room temperatures for several weeks.
Long-Term Storage
For longer term storage (several months to years) it is more preferable to store peptides in a freezer at -80C (-112F). When storing peptides for months or even years, freezing is optimal in order to preserve the peptide's stability.
Note: Avoid repeated freeze-thaw cycles. This can increase the peptide's susceptibility to degradation. Also, frost-free freezers should be avoided to store peptides, as temperatures can fluctuate widely during defrosting cycles.
Preventing Oxidation and Moisture Contamination
It is imperative to avoid contaminating peptides with both air and moisture. Moisture contamination is especially prone to occur when using a peptide immediately after withdrawing it from the freezer. To prevent uptake of moisture from the air on the cold surface of the peptide or on the inside of its container, allow the peptide to come to room temperature before opening.
It is also crucially important to minimize a peptide's exposure to the air. After the required amount of peptide has been removed, resealing the container under an atmosphere of dry, inert gas (such as nitrogen or argon) will minimize the potential for the remaining peptide to become oxidized.
Storing Peptides In Solution
The shelf life of peptide solutions is far less than that of lyophilized peptides, and peptides stored in solution are also vulnerable to bacterial degradation. Peptides containing Cys, Met, Trp, Asp, Gln, and N-terminal Glu in their sequences have especially short shelf lives when in solution.
Nevertheless, if peptides absolutely must be stored in solution, sterile buffers at pH 5-6 should be used. Peptide solutions are generally stable for up to 30 days when refrigerated at 4C (39F), but those peptides with inherent instability should be kept frozen when not in use.
Summary Checklist
- Store peptide in a cold, dry, dark place.
- Avoid repeated freezing and thawing of peptide.
- Avoid overexposure to the air and light.
- Avoid storing peptides in solution long term.
- Aliquot peptide into separate vials according to experimental requirements.