Peptide Purity
Notice
ALL ARTICLES AND PRODUCT INFORMATION PROVIDED ON THIS WEBSITE ARE FOR INFORMATIONAL AND EDUCATIONAL PURPOSES ONLY. The products offered on this website are furnished for in-vitro studies only. In-vitro studies (Latin: in glass) are performed outside of the body. These products are not medicines or drugs and have not been approved by the FDA to prevent, treat or cure any medical condition, ailment or disease. Bodily introduction of any kind into humans or animals is strictly forbidden by law.
How is Peptide Purity Achieved and Verified?
At Pure U.S. Peptides, published certificates report HPLC purity where tested; most published lots test 99%+. Published certificates come from third-party laboratories. Across the industry, peptide purity and identity are typically verified with two analytical methods: high-performance liquid chromatography and mass spectrometry analysis.
HPLC Analysis
High performance liquid chromatography, or HPLC, is a scientific technique used to separate, identify, and quantify each component in a mixture. It is a superior process that allows highly accurate peptide testing to be accomplished.
Mass Spectrometry
Mass spectrometry (MS) is a technique used to measure masses within a sample by ionizing chemical species and sorting the ions based on their mass to charge ratio. The results are plotted with the ion signal as a function of the mass to charge ratio.
Both methods are highly accurate peptide testing techniques: HPLC measures purity, while mass spectrometry confirms identity. Our published certificates report HPLC purity where tested, from third-party laboratories, and identity where the lab tested it.
What is the Recommended Peptide Purity Level?
Pure U.S. Peptides publishes certificates that report HPLC purity where tested; most published lots test 99%+. However, preparations of peptides synthesized for research by many other manufacturers can vary widely in purity. Occasionally, researchers can wonder what the minimum acceptable level of peptide purity is for their given purpose. Generally, the higher the peptide purity level, the more favorable the preparation; critically, for certain applications (such as in vitro studies or clinical trials), only exceedingly pure peptides will be appropriate (greater than 98% purity).
| Purity Level | Recommended Applications |
|---|---|
| Highly Pure (>95%) |
|
| Mid-range (>85%) |
|
| Lower Purity (>70%) |
|
Those peptides with greater purity are appropriate for use in an application with a lower minimum acceptable level of purity. Pure U.S. Peptides publishes certificates that report HPLC purity where tested, so researchers can compare a lot against the requirements above; most published lots test 99%+.