Storing Glow 70mg: Best Practices
Recommended Laboratory Storage Conditions — GLOW Blend (70 mg)
Lyophilized Powder: Store at -20°C for long-term stability (up to 24 months). White-to-pale-blue powder; the faint blue tint reflects the GHK-Cu copper complex (50 mg fraction). Hygroscopic — keep tightly sealed under inert gas (N₂ or Argon).
Reconstituted Solution: Store aliquots at -80°C for up to 6 months. Refrigerated (2–8°C) solutions should be used within 14 days due to BPC-157 and TB-500 hydrolysis kinetics. Reconstitute with bacteriostatic water or sterile saline using gentle swirling — do not vortex or shake aggressively.
pH Stability: Stable in the range of pH 5.0–7.0. Below pH 4 or above pH 9, GHK-Cu may dissociate and release free copper, which can catalyze oxidative degradation of BPC-157 and TB-500.
Critical Incompatibilities: Do not co-formulate with EDTA, carnosine, Vitamin C, or other strong chelators — these strip Cu(II) from GHK-Cu and abolish its activity.
Component Purity: ≥98% per peptide by HPLC; identity by mass spectrometry; TFA-free preferred for biological-grade research.
Handling: Allow vials to warm to room temperature before opening to prevent moisture condensation onto the lyophilized cake. Standard aseptic technique. Discard any solution that appears cloudy or contains particulate matter.
References
- Pickart L, Margolina A. Regenerative and Protective Actions of the GHK-Cu Peptide in the Light of the New Gene Data. International Journal of Molecular Sciences. 2018;19(7):1987.
- Sikiric P, et al. Stable Gastric Pentadecapeptide BPC 157, Robert's Stomach Cytoprotection. Current Pharmaceutical Design. 2020;26(25):3024-3044.
- Goldstein AL, Hannappel E, Sosne G, Kleinman HK. Thymosin β4: a multi-functional regenerative peptide. Expert Opinion on Biological Therapy. 2012;12(1):37-51.
- Pickart L, Vasquez-Soltero JM, Margolina A. GHK-Cu may Prevent Oxidative Stress in Skin. Cosmetics. 2015;2(3):236-247.
- Hsieh MJ, et al. Therapeutic potential of pro-angiogenic BPC157 is associated with VEGFR2 activation. Journal of Molecular Medicine. 2017;95(3):323-333.
- Xing Y, Ye Y, Zuo H, Li Y. Progress on the Function and Application of Thymosin β4. Frontiers in Endocrinology. 2021;12:767785.
- U.S. Food and Drug Administration. Certain Bulk Drug Substances for Use in Compounding. FDA.gov. Updated 2023.
- World Anti-Doping Agency. The 2025 Prohibited List. WADA. January 1, 2025.
- Pickart L, Vasquez-Soltero JM, Margolina A. GHK and DNA: Resetting the human genome to health. BioMed Research International. 2014;2014:151479.
- Pickart L, Vasquez-Soltero JM, Margolina A. GHK Peptide as a Natural Modulator of Multiple Cellular Pathways. BioMed Research International. 2015;2015:648108.
- Maquart FX, Pickart L, et al. Stimulation of collagen synthesis by GHK-Cu. FEBS Letters. 1988;238(2):343-346.
- Schlosser N. BPC-157: A Polyproline II Helix Engages SH3 Domains of Src Family Kinases. 2025.
- Chang CH, et al. BPC 157 Enhances the Growth Hormone Receptor Expression in Tendon Fibroblasts. Molecules. 2014;19(12):19066-19077.
- Bock-Marquette I, et al. Thymosin beta4 activates integrin-linked kinase and promotes cardiac cell migration. Nature. 2004;432(7016):466-472.
- Sosne G, Qiu P, Goldstein AL, Wheater M. Biological activities of thymosin beta 4. The FASEB Journal. 2010;24(7):2144-2151.
- Bock-Marquette I, et al. Thymosin beta-4 denotes new directions for anti-aging therapies. International Immunopharmacology. 2023;116:109741.
- Canapp SO Jr, et al. Topical tripeptide-copper complex on healing of ischemic open wounds. Veterinary Surgery. 2003;32(6):515-523.
- Philp D, et al. Thymosin β4 and a synthetic peptide containing its actin-binding domain promote dermal wound repair. Wound Repair and Regeneration. 2003;11(1):19-24.
- Staresinic M, et al. BPC 157 accelerates healing of transected rat Achilles tendon. Journal of Orthopaedic Research. 2003;21(6):976-983.
- Badenhorst T, et al. Effects of GHK-Cu on MMP and TIMP Expression. Journal of Aging Science. 2016;4(3):166.
- Smart N, et al. Thymosin β4 induces adult epicardial progenitor mobilization and neovascularization. Nature. 2007;445(7124):177-182.
- Kuceki G, et al. Enhanced hair regrowth with minoxidil-dutasteride-copper peptides for androgenetic alopecia. JAAD International. 2025;20:38-40.
- Goldstein AL, et al. Thymosin β4: actin-sequestering protein moonlights to repair injured tissues. Trends in Molecular Medicine. 2005;11(9):421-429.
- Sever M, et al. BPC 157 counteracts liver fibrosis. Journal of Physiology and Pharmacology. 2019;70(3):391-400.
- Shah R, et al. Thymosin β4 Prevents Oxidative Stress, Inflammation, and Fibrosis in Liver Injury. Oxidative Medicine and Cellular Longevity. 2018;2018:9630175.
- Sosne G, Dunn SP, Kim C. Thymosin β4 Improves Severe Dry Eye in a Phase 2 Randomized Trial. Cornea. 2015;34(5):491-496.
Related Research Questions
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